Fluorophore-conjugated gold nanoparticles (GNPs) are in high demand for the development of diagnostic tools for diseases, including cancer. We have described a method of covalent attachment of fluorescein to GNPs with a diameter of 12.7 (GNP-13) and 6.1 nm (GNP-6) via a linker –PEG71-NH-(CH2)6-NH–, allowing to separate the GNP and fluorophore in space to a distance necessary to overcome the effect of fluorescence quenching by the GNP surface. The obtained fluorescent GNP conjugates can be effectively used for biovisualization of tumor tissues in vivo.
Author Biographies
Gorbunova Ekaterina Andreevna, Institute of Chemical Biology and Fundamental Medicine SB RAS
First category engineer of the Laboratory of Biomedical ChemistryORCID: 0000-0002-5391-5322 Researcher ID: ABC-7310-2021 Scopus Author ID: 58454212800
Epanchintseva Anna Valerjevna, Institute of Chemical Biology and Fundamental Medicine SB RAS
Candidate of Chemical Sciences, Researcher of the Laboratory of Biomedical ChemistryORCID: 0000-0003-4720-4564 Researcher ID: R-4095-2017 Scopus Author ID: 57193308282
Pyshnaya Inna Alekseevna, Institute of Chemical Biology and Fundamental Medicine SB RAS
Candidate of Chemical Sciences, Senior Researcher of the Laboratory of Biomedical ChemistryORCID: 0000-0002-7559-2376 Researcher ID: F-9519-2013 Scopus Author ID: 6603033141
